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Anti-inflammatory effect of liposome-encapsulated and free from diclofenac in rats / Goh Jun Zheng.

By: Goh, Jun Zheng.
Material type: materialTypeLabelBookPublisher: 2011. Description: 46 leaves : ill. ; 30cm.Other title: Kesan anti- inflamasi diclofenac bersalut liposom dan diclofenac bebas ke atas tikus.Dissertation note: Project paper (B.S.(Biomedical Sciences)) - Universiti Putra Malaysia, 2011. Summary: Diclofenac, a non-steroidal anti-inflammatory drug (NSAID), which has the function of reducing inflammation and pain. Liposome is a type of microcapsule, enclose liquid compartments with their multimellar structure consisting of phospholipid bilayer. This study is conducted to evaluate the anti-inflammatory efficacy between liposome-encapsulated and free form diclofenac in rat. Diclofenac-loaded liposome samples are prepared using propilosome method by mixing 1 gram of diclofenac compound with 20µl of 60mg/ml DMSO. The acute, sub-acute and chronic anti-inflammatory effects of both liposome-encapsulated and free diclofenac were evaluated using carrageenan-induced paw edema, formalin-induced paw edema and cotton-pellet-induced granuloma tests on male Spague-Dawley rats respectively. For each test the animals were randomly divided into two groups of 24 , each group received either free diclofenac or liposome-encapsulated diclofenac. Each group was further divided into 4 subgroups with 6 animals each which received different doses of drug (0, 0.2, 2, 20 mg/kg). All data were subjected to one-way ANOVA followed by Duncan’— new multiple range test among groups. For carrageenan-induced paw edema test, significant reduction (p<0.05) in paw volume was observed in 3rd and 4th hour for treatment group 0.2, 2, 20 mg/kg for both liposome-encapsulated and free form diclofenac. Group 2 and 20 mg/kg for liposome-encapsulated diclofenac shown significant (P<0.05) paw volume reduction compared to free drug of same dosage group in 3rd and 4th hour respectively. In formalin test, the anti-inflammatory effect, which characterized by the reduction of paw-licking time in rats, was observed in both early and late phases. However significant (P<0.05) reduction in paw-licking time only observed in late phase for both liposome-encapsulated and free form diclofenac (2 and 20 mg/kg) with the percentage of inhibition of 28.62, 60.17% for free form diclofenac and 31.45, 78.84 % for liposome-encapsulated diclofenac. In cotton-pellet-induced granuloma test, anti-inflammatory effect, which characterized by the reduction in size of granuloma formed was observed in both transudative and granuloma weight. Treatment group 20mg/kg free form diclofenac shown Significant result (P<0.05) in both transudative and granuloma weight with percentage of inhibition of 42.93 and 49.26% respectively. For liposome-encapsulated diclofenac, treatment group 2 and 20mg/kg diclofenac shown significant result (P<0.05) in both transudative and granuloma weight with percentage of inhibition of 27.42, 36.93 and 48.43, 63.55 % respectively. Anti-inflammatory tests shown Liposome-encapsulated diclofenac exhibit higher efficacy effects than free form diclofenac when orally administratered into experimental rats. Thus, it is concluded that the encapsulation of diclofenac by liposome enhance the therapeutic effect of drug in anti-inflammatory assays.
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Project paper (B.S.(Biomedical Sciences)) - Universiti Putra Malaysia, 2011.

Diclofenac, a non-steroidal anti-inflammatory drug (NSAID), which has the function of reducing inflammation and pain. Liposome is a type of microcapsule, enclose liquid compartments with their multimellar structure consisting of phospholipid bilayer. This study is conducted to evaluate the anti-inflammatory efficacy between liposome-encapsulated and free form diclofenac in rat. Diclofenac-loaded liposome samples are prepared using propilosome method by mixing 1 gram of diclofenac compound with 20µl of 60mg/ml DMSO. The acute, sub-acute and chronic anti-inflammatory effects of both liposome-encapsulated and free diclofenac were evaluated using carrageenan-induced paw edema, formalin-induced paw edema and cotton-pellet-induced granuloma tests on male Spague-Dawley rats respectively. For each test the animals were randomly divided into two groups of 24 , each group received either free diclofenac or liposome-encapsulated diclofenac. Each group was further divided into 4 subgroups with 6 animals each which received different doses of drug (0, 0.2, 2, 20 mg/kg). All data were subjected to one-way ANOVA followed by Duncan’— new multiple range test among groups. For carrageenan-induced paw edema test, significant reduction (p<0.05) in paw volume was observed in 3rd and 4th hour for treatment group 0.2, 2, 20 mg/kg for both liposome-encapsulated and free form diclofenac. Group 2 and 20 mg/kg for liposome-encapsulated diclofenac shown significant (P<0.05) paw volume reduction compared to free drug of same dosage group in 3rd and 4th hour respectively. In formalin test, the anti-inflammatory effect, which characterized by the reduction of paw-licking time in rats, was observed in both early and late phases. However significant (P<0.05) reduction in paw-licking time only observed in late phase for both liposome-encapsulated and free form diclofenac (2 and 20 mg/kg) with the percentage of inhibition of 28.62, 60.17% for free form diclofenac and 31.45, 78.84 % for liposome-encapsulated diclofenac. In cotton-pellet-induced granuloma test, anti-inflammatory effect, which characterized by the reduction in size of granuloma formed was observed in both transudative and granuloma weight. Treatment group 20mg/kg free form diclofenac shown Significant result (P<0.05) in both transudative and granuloma weight with percentage of inhibition of 42.93 and 49.26% respectively. For liposome-encapsulated diclofenac, treatment group 2 and 20mg/kg diclofenac shown significant result (P<0.05) in both transudative and granuloma weight with percentage of inhibition of 27.42, 36.93 and 48.43, 63.55 % respectively. Anti-inflammatory tests shown Liposome-encapsulated diclofenac exhibit higher efficacy effects than free form diclofenac when orally administratered into experimental rats. Thus, it is concluded that the encapsulation of diclofenac by liposome enhance the therapeutic effect of drug in anti-inflammatory assays.

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